Quantitative analysis by UV-Vis absorption spectroscopy of amino groups attached to the surface of carbon-based nanoparticles

International Conference on Advanced Materials for Better Future 2017

Saraswati, T.E.^1 ; Astuti, A.R.^1 ; Rismana, N.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36 A, Kentingan, Surakarta

57126, Indonesia^1

关键词: Absorbance intensity;    Absorption peaks;    Fourier transform infrared;    FT-IR spectrum;    Functionalizations;    Performance signals;    UV-Vis absorption spectroscopy;    UV-vis spectroscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/333/1/012027/pdf
DOI  :  10.1088/1757-899X/333/1/012027

来源: IOP

【 摘 要 】

Carbon-based nanoparticles must be modified due to their wide array of applications, especially when they are used as biomaterials. After modifying, quantitative analysis of the functional group is essential to evaluate a number of the available functional groups applied for further functionalization. In this study, we modified the carbon-based nanoparticles by amino group using submerged arc discharge in different liquids. The attached amino groups were then characterised and quantified by UV-Vis spectroscopy. This amino group functionalization was also confirmed by Fourier transform infrared (FTIR) spectra. The FTIR spectra of amine-modified nanoparticles show the definitive absorption peaks of N - H amine, C - H, C=O, C - N and Fe - O at 3418.97; 3000-2850; 1700-1600; 1400-1100; and 480-550 cm-1, respectively. The amine groups have different performance signals between the amine-modified and unmodified nanoparticles. The FTIR spectra results were correlated with the UV-Vis absorption spectroscopy method using acidic methyl orange. The UV-Vis absorption spectroscopy shows that the absorbance of methyl orange represented to amino groups number was 1.3 times higher when the pH of the solution was increased. The absorbance intensity was then used to estimate the quantity of amine groups attached.