Spider Silk Processing for Spidroin Recovery from Crossopriza Lyoni Web

Malaysian Technical Universities Conference on Engineering and Technology 2017

Mohtar, J.A.^1 ; Ooi, W.L.^1 ; Yusuf, F.^2

Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis, Uniciti Alam Campus, Sungai Chuchuh, Padang Besar, Perlis

02100, Malaysia^1

Department of Biotechnology Engineering, Kulliyah of Engineering, International Islamic University Malaysia, Gombak, Malaysia^2

关键词: Effect of temperature;    Extraction process;    Incubation time;    Optimal conditions;    Physicomechanical properties;    Protein concentrations;    Protein recovery;    Statistical optimization;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/318/1/012016/pdf
DOI  :  10.1088/1757-899X/318/1/012016

来源: IOP

【 摘 要 】

Spider silk is a potential biomaterial that can be used in various applications for its outstanding physicomechanical properties attributed by the spidroin composition. Efforts for commercializing spider silks have been mainly focused on the characterization of spidroins from the Entelegyne spiders for exceptional fibre construction. Hence, studies on silk proteins from the Haplogyne species remain neglected. The aim of this study is to isolate spidroin from Crossopriza lyoni web. Silk processing involved the pretreatment of fibres for the shell layer removal from the surface. A screening study was conducted to analyze the effect of temperature, incubation time and agitation speed on spidroin extraction using Ajisawa's reagent by OFAT analysis followed by statistical optimization of the extraction process via RSM for maximal protein recovery. All parameters exerted significant effect on spidroin recovery (p