Improved design of a cone-shaped rotating disk for shear force loading in a cell culture plate

8th TSME-International Conference on Mechanical Engineering

Keawprachum, Boonrit^1 ; Limjeerajarus, Nuttapol^2 ; Limjeerajarus, Chalida Nakalekha^3 ; Srisungsitthisunti, Pornsak^1

Production Engineering Program, Faculty of Engineering, King Mongkut's University of Technology North Bangkok, 1518 Pracharat I Rd., Wongsawang, Bangsue, Bangkok

10800, Thailand^1

Research Center for Advanced Energy Technology, Faculty of Engineering, Thai-Nichi Institute of Technology, 1771/1 Pattanakarn, Suan Luang, Bangkok

10250, Thailand^2

Department of Physiology, Faculty of Dentistry, Chulalongkorn University, 34 Henri Dunant Rd, Pathumwan, Bangkok

10330, Thailand^3

关键词: Cell culture plates;    Cone-shaped;    FLUENT software;    Improved designs;    Shear force;    Tilt angle;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/297/1/012025/pdf
DOI  :  10.1088/1757-899X/297/1/012025

来源: IOP

【 摘 要 】

In our previous study, a cone-shaped rotating disk had been designed and proposed for generating shear force on the cell in a cell culture plate. This study aims to improve the design of the rotating disk that could provide a better uniformity of shear stress distribution. The top of the cone was designed to be trimmed off to obtain a flat head area. The effect of tilt angle (θ) was numerically studied using computational fluid dynamics (CFD) technique in ANSYS-Fluent software. The results revealed that for 500 rpm, the new designed rotating disk with a height of cone-shaped top to the plate bottom h = 1 mm and θ = 25° provided the best uniformity of 0.820 which was better than that of the previously designed.