Optimization of preparation of NDV F Gene encapsulated in N-2-HACC-CMC nanoparticles

2nd International Conference on New Material and Chemical Industry

材料科学;化学工业

Li, S.S.^1 ; Zhang, Y.^1 ; Zhao, K.^1,3 ; Wang, X.H.^2,3

Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin

150080, China^1

Heilongjiang University Library, Harbin

150080, China^2

School of Biological Science and Technology, University of Jinan, Jinan

250022, China^3

关键词: Ammonium chloride;    Biodegra-dable materials;    Delivery carriers;    Entrapment efficiency;    Loading capacities;    N ,o-carboxymethyl chitosans;    Preparation conditions;    Smooth surface;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/292/1/012022/pdf
DOI  :  10.1088/1757-899X/292/1/012022

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

In this study, the biodegradable materials N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) and N, O-carboxymethyl chitosan (CMC) are used as delivery carrier for the pVAX I-F(o)-C3d6. The optimal preparation condition is as follows: Concentration of N-2-HACC is 1.0 mg/ml, concentration of CMC is 0.85 mg/ml, concentration of pVAX I-F(o)-C3d6 is 100 μg ml. The results show that the prepared N-2-HACC-CMC/pFDNA NPs have regular round shape, smooth surface and good dispersion, the particle size is 310 nm, Zeta potential is 50 mV, the entrapment efficiency is 92 %, the loading capacity is 51 % (n=3).