Fatty Acid Analysis of Lipid Extracted from Rats by Gas Chromatography-Mass Spectrometry Method

2nd Annual Applied Science and Engineering Conference

工业技术;自然科学

Utami, P.I.^1 ; Rahayu, W.S.^1 ; Nugraha, I.^1 ; Rochana, A.N.^1

Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Indonesia^1

关键词: Carbon atoms;    Fatty acid composition;    Fatty acid methyl ester;    Gas chromatography-mass spectrometry methods;    Hexadecanoic acid;    Methyl esters;    Octadecanoic acids;    Tetradecanoic acid;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/288/1/012115/pdf
DOI  :  10.1088/1757-899X/288/1/012115

来源: IOP

【 摘 要 】

The purpose of this study was to identify fatty acid composition in fat extracted from rats using Gas Chromatography Mass Spectrometry (GC-MS) method. The fatty acid composition was determined as the methyl esters of fatty acids. Fat was extracted with chloroform-methanol solvent. Fatty acid methyl esters were prepared by saponification with base and followed by BF3-catalyzed methylation. Thirty compounds were identified, representing about 100 % of the total extracted lipids as measured by GC peak areas. The major constituents were 9-Octadecenoic acid; Hexadecanoic acid; 9,12-Octadecadienoic acid; Octadecanoic acid; 9-Hexadecenoic acid; Tetradecanoic acid; and Methyl arachidonate. The major constituents were fatty acids with chain lengths from 15 to 21 carbon atoms (mainly C17 and C19).