2nd Annual Applied Science and Engineering Conference | |
Fatty Acid Analysis of Lipid Extracted from Rats by Gas Chromatography-Mass Spectrometry Method | |
工业技术;自然科学 | |
Utami, P.I.^1 ; Rahayu, W.S.^1 ; Nugraha, I.^1 ; Rochana, A.N.^1 | |
Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Indonesia^1 | |
关键词: Carbon atoms; Fatty acid composition; Fatty acid methyl ester; Gas chromatography-mass spectrometry methods; Hexadecanoic acid; Methyl esters; Octadecanoic acids; Tetradecanoic acid; | |
Others : https://iopscience.iop.org/article/10.1088/1757-899X/288/1/012115/pdf DOI : 10.1088/1757-899X/288/1/012115 |
|
来源: IOP | |
【 摘 要 】
The purpose of this study was to identify fatty acid composition in fat extracted from rats using Gas Chromatography Mass Spectrometry (GC-MS) method. The fatty acid composition was determined as the methyl esters of fatty acids. Fat was extracted with chloroform-methanol solvent. Fatty acid methyl esters were prepared by saponification with base and followed by BF3-catalyzed methylation. Thirty compounds were identified, representing about 100 % of the total extracted lipids as measured by GC peak areas. The major constituents were 9-Octadecenoic acid; Hexadecanoic acid; 9,12-Octadecadienoic acid; Octadecanoic acid; 9-Hexadecenoic acid; Tetradecanoic acid; and Methyl arachidonate. The major constituents were fatty acids with chain lengths from 15 to 21 carbon atoms (mainly C17 and C19).
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
Fatty Acid Analysis of Lipid Extracted from Rats by Gas Chromatography-Mass Spectrometry Method | 653KB | download |