Determination of Betaine in Forsythia Suspensa by High Performance Capillary Electrophoresis

1st International Conference on Frontiers of Materials Synthesis and Processing

材料科学;化学

Liu, Haixing^1 ; Dong, Guoliang^1 ; Wang, Lintong^1

Chemistry and Chemical and Environmental Engineering College, Weifang University, Weifang

261061, China^1

关键词: Borax solution;    Buffer solutions;    Capillary columns;    Constant voltage;    Correlation coefficient;    High performance capillary electrophoresis;    Pressure time;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/274/1/012076/pdf
DOI  :  10.1088/1757-899X/274/1/012076

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

This paper presents the determination of betaine content of Forsythia suspensa by high performance capillary electrophoresis (HPCE) method. The borax solution was chosen as buffer solution, and its concentration was 40 mmol with capillary column (75μm×52/60cm) at a constant voltage of 20kV and injecting pressure time of 10s at 20°C. Linearity was kept in the concent ration range of 0.0113-1.45mg•ml-1 of betaine with correlation coefficient of 0.999. The recovery was in the range of 97%-117% (n=5), The content of betaine was 281.5 mg•g-1and RSD value of 9.6% (n=6) in Forsythia suspensa. This method has the advantage of rapid, accurate and good repeatability in separation and determination of betaine in Forsythia suspensa.