Production of fibrinolytic protease from Streptomyces lusitanus isolated from marine sediments

14th International Conference on Science, Engineering and Technology

自然科学;工业技术

Sudeshwarma, S.^1 ; Merlyn Keziah, S.^1 ; Subathra Devi, C.^1

School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India^1

关键词: Fibrinolytic enzymes;    Marine sediments;    Marine streptomyces;    Maximum productivity;    Nitrogen sources;    Optimized parameter;    Purified enzyme;    Streptomyces lusitanus;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022048/pdf
DOI  :  10.1088/1757-899X/263/2/022048

来源: IOP

【 摘 要 】

This study aim was to isolate, screen, characterize and optimize marine Streptomyces for fibrinolytic enzyme production. The potent actinomycete isolate was subjected to optimization. The parameters for optimization included pH, temperature, carbon, nitrogen sources. The crude supernatant produced was purified using size exclusion gel filtration chromatography. The optimized parameters for maximum productivity were found to be pH 7, 37°C, maltose and peptone respectively. The molecular weight of the purified enzyme was found to be 21kDa.