Total alkaloid content in various fractions of Tabernaemonata sphaerocarpa Bl. (Jembirit) leaves

International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products

Salamah, N.^1 ; Ningsih, D.S.^1

Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia^1

关键词: Alkaloid compounds;    Anti-inflammatories;    Bromocresol greens;    Cytotoxic activities;    Ethanolic extracts;    Spectro-photometric method;    T. sphaerocarpa Bl;    Visible spectrophotometries;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/259/1/012017/pdf
DOI  :  10.1088/1757-899X/259/1/012017

来源: IOP

【 摘 要 】

Tabernaemontana sphaerocarpa Bl. (Jembirit) is one of the Apocynaceae family plants containing alkaloid compound. Traditionally, it is used as an anti-inflammatory medicine. It is found to have a new bisindole alkaloid compound that shows a potent cytotoxic activity in human cancer. This study aimed to know the total alkaloid content in some fractions of ethanolic extract of T. sphaerocarpa Bl. leaf powder was extracted by maceration method in 70% ethanol solvent. Then, the extract was fractionated in a separatory funnel using water, ethyl acetate, and hexane. The total alkaloid content in each fraction was analyzed with visible spectrophotometric methods based on the reaction with Bromocresol Green (BCG). The total alkaloids in water fraction and ethyl acetate fraction were (0.0312±0.0009)% and (0.0281±0.0014)%, respectively. Meanwhile, the total alkaloid content in hexane was not detected. The statistical analysis, performed in SPSS, resulted in a significant difference between the total alkaloids in water fraction and ethyl acetate fraction. The total alkaloid in water fraction of T. sphaerocarpa Bl. was higher than the one in ethyl acetate fraction.