High-speed precise cell patterning by pulsed electrohydrodynamic jet printing

Scanning Probe Microscopy 2017

Makaev, A.V.^1 ; Mingaliev, E.A.^1 ; Karpov, V.R.^1 ; Zubarev, I.V.^1 ; Ya Shur, V.^1 ; El'Kina, O.S.^1

Ural Federal University, Ekaterinburg

620002, Russia^1

关键词: Cell-patterning;    Drop-on-demand printing;    Droplet generation;    Electrohydrodynamic jet printings;    Electrohydrodynamic printings;    Nutrient medium;    Optimal printing;    Situ visualization;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/256/1/012013/pdf
DOI  :  10.1088/1757-899X/256/1/012013

来源: IOP

【 摘 要 】

The generation of micro-droplets of nutrient medium with living cells by pulsed electrohydrodynamic printing has been studied. In-situ visualization by high-speed camera made it possible to measure the characteristic times of droplet generation process and to determine the optimal printing parameters. Maximal frequency of stable generation was achieved at 700 Hz. This technique was applied successfully for drop-on-demand printing of culture medium with live HeLa cells and yeasts.