Combination of atomic force microscopy and mass spectrometry for the detection of target protein in the serum samples of children with autism spectrum disorders

Scanning Probe Microscopy 2017

Kaysheva, A.L.^1 ; Pleshakova, T.O.^1 ; Kopylov, A.T.^1 ; Shumov, I.D.^1 ; Iourov, I.Y.^2 ; Vorsanova, S.G.^2 ; Yurov, Y.B.^2 ; Ziborov, V.S.^3 ; Archakov, A.I.^1 ; Ivanov, Y.D.^1

Institute of Biomedical Chemistry, Moscow, Russia^1

Mental Health Research Center, Moscow, Russia^2

Joint Institute for High Temperatures RAS, Moscow, Russia^3

关键词: Autistic disorders;    Biological samples;    Children with autisms;    Quantitative detection;    Serum samples;    Target proteins;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/256/1/012015/pdf
DOI  :  10.1088/1757-899X/256/1/012015

来源: IOP

【 摘 要 】

Possibility of detection of target proteins associated with development of autistic disorders in children with use of combined atomic force microscopy and mass spectrometry (AFM/MS) method is demonstrated. The proposed method is based on the combination of affine enrichment of proteins from biological samples and visualization of these proteins by AFM and MS analysis with quantitative detection of target proteins.