The SPR detection of Salmonella enteritidis in food using aptamers as recongnition elements

2017 2nd International Seminar on Advances in Materials Science and Engineering

Di, W.T.^1 ; Du, X.W.^2 ; Pan, M.F.^1 ; Wang, J.P.^1

Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin

300457, China^1

Dalian Nationalities University, Dalian

116600, China^2

关键词: Detection methods;    Experimental conditions;    Experimental scheme;    Immune recognition;    Low detection limit;    Regenerating solutions;    Salmonella enteritidis;    Simple operation;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/231/1/012114/pdf
DOI  :  10.1088/1757-899X/231/1/012114

来源: IOP

【 摘 要 】

In this experiment, a fast, accurate, non-destructive, unmarked and simple-operation detection method for Salmonella enteritidis in food was established by the BI-3000 plasma resonance biosensor (SPR). This article establishes a method of using nucleic acid aptamer as immune recognition element in SPR which can be employed to detect Salmonella enteritidis in food for the first time. The experimental conditions were screened and the experimental scheme was validated and applied. The best flow rate was 5μL/min, the best concentration of the aptamers was 180mM, and the best regenerating solution was the 20mM NaOH. This method had almost no cross-reactivity. Besides, we established a standard curve of Salmonella enteritidis and SPR signal, with the detection limit of 2 cfu/mL. Finally, we tested the samples of chicken, pork, shrimp and fish purchased from supermarkets. The method has the advantages of short time, low detection limit and easy operation, which can be used for a large number of food samples.