Isolation and Identification of Concrete Environment Bacteria

Soft Soil Engineering International Conference 2015

Irwan, J.M.^1 ; Anneza, L.H.^1 ; Othman, N.^2 ; Husnul, T.^3 ; Alshalif, A.F.^1

Research Centre for Susitainable Construction, Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia, Batu Pahat Johor

86400, Malaysia^1

Micropollutant Research Centre, Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia, Batu Pahat Johor

86400, Malaysia^2

School of Industrial Technology, University Sains Malaysia, Penang, Malaysia^3

关键词: 16S rDNA gene sequencing;    Bacteria identifications;    Bioconcrete;    Concrete environments;    Effective microorganisms;    Enterococus faecalis;    Enzymatic pathways;    Isolation and identification;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/136/1/012088/pdf
DOI  :  10.1088/1757-899X/136/1/012088

来源: IOP

【 摘 要 】

This paper presents the isolation and molecular method for bacteria identification through PCR and DNA sequencing. Identification of the bacteria species is required in order to fully utilize the bacterium capability for precipitation of calcium carbonate in concrete. This process is to enable the addition of suitable catalyst according to the bacterium enzymatic pathway that is known through the bacteria species used. The objective of this study is to isolate, enriched and identify the bacteria species. The bacteria in this study was isolated from fresh urine and acid mine drainage water, Kota Tinggi, Johor. Enrichment of the isolated bacteria was conducted to ensure the bacteria survivability in concrete. The identification of bacteria species was done through polymerase chain reaction (PCR) and rRDNA sequencing. The isolation and enrichment of the bacteria was done successfully. Whereas, the results for bacteria identification showed that the isolated bacteria strains are Bacillus sp and Enterococus faecalis.