The effect of CPP-ACP-propolis chewing gum on calcium and phosphate ion release on caries-active subjects' saliva and the formation of Streptococcus mutans biofilm

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Hasnamudhia, F.^1 ; Bachtiar, E.W.^1 ; Sahlan, M.^2 ; Soekanto, S.A.^1

Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia^2

关键词: Atomic absorption spectrophotometer;    Calcium levels;    Chewing simulation;    Phosphate levels;    S. mutans biofilms;    Streptococcus mutans;    Streptococcus mutans biofilms;    Ultraviolet visible spectrophotometer;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012137/pdf
DOI  :  10.1088/1742-6596/884/1/012137

学科分类：卫生学

来源: IOP

【 摘 要 】

The aim of this study was to analyze the effect of CPP-APP and propolis wax if they are combined in a chewing gum formulation, observed from the calcium and phosphate ion level released by CPP-ACP and the emphasis of Streptococcus mutans mass in the biofilm by propolis wax on caries-active subjects' saliva. Chewing gum simulation was done in vitro on 25 caries-active subjects' saliva using five concentrations of chewing gum (0% propolis + 0% CPP-ACP, 0% propolis + CPP-ACP, 2% propolis + CPP-ACP, 4% propolis + CPP-ACP, and 6% propolis + CPP-ACP) and was then tested using an atomic absorption spectrophotometer to analyze calcium ion levels, an ultraviolet-visible spectrophotometer to analyze phosphate ion levels, and a biofilm assay using crystal violet to analyze the decline in biofilm mass. After the chewing simulation, calcium ion levels on saliva+gum eluent increased significantly compared to the saliva control, with the highest calcium level released by CPP-ACP + 2% propolis chewing gum. There was an insignificant phosphate level change between the saliva control and saliva+gum eluent. There was also a significant decline of S. mutans biofilm mass in the saliva+gum eluent, mostly by the CPP-ACP chewing gum and CPP-ACP + 6% propolis. The CPP-ACP-propolis chewing gum simulation generated the largest increase in calcium and phosphate ion level and the largest decline in S. mutans biofilm mass.