Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Verisqa, F.^1 ; Triaminingsih, S.^2 ; Corputty, J.E.M.^1

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

Department of Dental Materials, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^2

关键词: Bone regeneration;    Collagen composites;    Compositional changes;    Energy dispersive spectroscopies (EDS);    Layer formation;    Maxillofacial reconstruction;    Nucleation sites;    Simulated body fluids;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012035/pdf
DOI  :  10.1088/1742-6596/884/1/012035

学科分类：卫生学

来源: IOP

【 摘 要 】

Hydroxyapatite (HA) formation is one of the most important aspects of bone regeneration. Because domestically made chitosan-hydroxyapatite-collagen composite scaffolding from crab shell and bovine bone and tendon has potential as a maxillofacial reconstruction material, the material's HA-forming ability requires evaluation. The aim of this research is to investigate chitosan-hydroxyapatite-collagen composite scaffold's potential as a maxillofacial reconstruction material by observing the scaffold's compositional changes. Scaffold specimens were immersed in 37°C simulated body fluid (SBF) for periods of 2, 4, 6, and 8 days. Scaffold composition was then evaluated by using energy dispersive spectroscopy (EDS). The calcium (Ca) and phosphorus (P) percentages of the scaffold were found to increase following SBF immersion. The high Ca/P ratio (3.82) on the scaffold indicated HA formation. Ion exchange played a significant role in the increased percentages of Ca and P, which led to new HA layer formation. The scaffold's HA acted as a nucleation site of Ca and P from the SBF, with collagen and chitosan as the scaffold's matrix. Chitosan-hydroxyapatite-collagen composite scaffold shows potential as a maxillofacial reconstruction material, since its composition favors HA formation.