The effectiveness of laser diode induction to Carica Papaya L. chlorophyll extract to be ROS generating in the photodynamic inactivation mechanisms for C.albicans biofilms

International Conference on Physical Instrumentation and Advanced Materials

物理学;材料科学

Astuty, S Dewi^1,3 ; Baktir, A.^2

Faculty of Science and Technology, Universitas Airlangga, Surabaya, East Java, Indonesia^1

Department of Chemistry, Universitas Airlangga, Surabaya, East Java, Indonesia^2

Department of Physics, Faculty of Science and Technology, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia^3

关键词: Albicans;    Anti-fungal;    Carica papaya;    In-situ techniques;    In-vitro;    Negative control;    Photodynamic inactivation;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/853/1/012026/pdf
DOI  :  10.1088/1742-6596/853/1/012026

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Research on the effectiveness of photo inactivation of C.albicans biofilms led by a-PDT system mediated by chlorophyll-diode-laser-induced was done. This research was done using in vitro technique in order to effectively determine chlorophyll extract of ROS-generated Carica Papaya L. using in situ technique. This technique induced laser diode on different dose and C. albicans with reduced degree. This research is a preliminary study in efforts to find anew sensitizer agent candidate made of chlorophyll extract and antifungal of Carica Papaya L. The effectiveness of eradication has been tested with MDA's content and OD of biomass biofilms as well as analyzed using ANOVA and Tukey Test (α=0.05). The characteristic of chlorophyll extract of Carica Papaya L. has maximum absorptions on blue areas (λmax= 420 nm) and red areas (λmax= 670 nm). The MIC value of Carica Papaya L.'schlorophyll extract against C. albicans planktonic and biofilms cell is 63.8 μM and 31.9 μM respectively. The result shows that treatment using laser which was combined with chlorophyll extract is more effective than that with laser only or chlorophyll extract only. The treatment using laser combined with chlorophyll extract obtained more than 65% (α=0.05) (more than that of negative control) for P2L1group with OD5950.915. The MDA's content showed that group of laser which was mediated with chlorophyll extract had larger values than group of laser or chlorophyll extract only.