Breast cancer cell targeted MR molecular imaging probe: Anti-MUC1 antibody-based magnetic nanoparticles

10th International Seminar on Medical Physics; 1st AMDI International Oncology Symposium

物理学;医药卫生

Khaniabadi, P. Moradi^1 ; Majid, A.M.S.A.^2 ; Asif, M.^2 ; Khaniabadi, B Moradi^3 ; Shahbazi-Gahrouei, D.^4 ; Jaafar, M.S.^1

School of Physics, University Science Malaysia, Pulau Penang

11800, Malaysia^1

EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang

11800, Malaysia^2

Child Growth and Development Research Centre, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran^3

Department of Medical Physics, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran^4

关键词: Atomic absorption spectroscopy;    Breast cancer cells;    Early-stage breast cancer;    Linear relationships;    Magnetic nano-particles;    Magnetic Resonance Imaging (MRI);    Superparamagnetic iron oxide nanoparticles;    Targeting efficacies;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/851/1/012014/pdf
DOI  :  10.1088/1742-6596/851/1/012014

学科分类：卫生学

来源: IOP

【 摘 要 】

Effective and specific diagnostic imaging techniques are important in early-stage breast cancer treatment. The objective of this study was to develop a specific breast cancer contrast agent for magnetic resonance imaging (MRI). In so doing, superparamagnetic iron oxide nanoparticles (SPIONs) were conjugated to C595 monoclonal antibody using EDC chemistry to produce nanoprobe with high relaxivity and narrow size (87.4±0.7 nm). To test the developed nanoprobe in vitro, assessments including Cell toxicity, targeting efficacy, cellular binding, and MR imaging were carried out. The results indicated that after 6 hrs incubation with MCF-7 cells at 200 to 25 μg Fe/ml doses, 76% to 16% T2reduction was obtained. The presence of iron localised in MCF-7 cells measured by atomic absorption spectroscopy (AAS) was about 9.95±0.09 ppm iron/cell at higher doses of nanoprobe. Moreover, a linear relationship between iron concentration of nontoxic SPION-C595 and T2relaxation times was observed. This study also revealed that developed nanoprobe might be used as a specific negative contrast agent for detecting breast cancer.