Quantitative Evaluation of Hard X-ray Damage to Biological Samples using EUV Ptychography

X-Ray Microscopy Conference 2016

Baksh, Peter^1 ; Odstrcil, Michal^1 ; Parsons, Aaron^2 ; Bailey, Jo^3 ; Deinhardt, Katrin^3 ; Chad, John E.^3 ; Brocklesby, William S.^1 ; Frey, Jeremy G.^4

Optoelectronics Research Centre, University of Southampton, United Kingdom^1

Diamond Light Source, Didcot

OX11 0DE, United Kingdom^2

Biological Sciences, Faculty of Natural and Environmental Sciences, University of Southampton, United Kingdom^3

Chemistry, Faculty of Natural and Environmental Sciences, University of Southampton, United Kingdom^4

关键词: Biological samples;    Coherent diffractive imaging;    Extreme ultraviolet sources;    High harmonic generation;    Hippocampal neurons;    Nanometre resolution;    Quantitative evaluation;    Synchrotron beamlines;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012034/pdf
DOI  :  10.1088/1742-6596/849/1/012034

来源: IOP

【 摘 要 】

Coherent diffractive imaging (CDI) has become a standard method on a variety of synchrotron beam lines. The high brilliance short wavelength radiation from these sources can be used to reconstruct attenuation and relative phase of a sample with nanometre resolution via CDI methods. However, the interaction between the sample and high energy ionising radiation can cause degradation to sample structure. We demonstrate, using a laboratory based high harmonic generation (HHG) based extreme ultraviolet (EUV) source, imaging a sample of hippocampal neurons using the ptychography method. The significant increase in contrast of the sample in the EUV light allows identification of damage induced from exposure to 7.3 keV photons, without causing any damage to the sample itself.