Multimodal imaging of the human knee down to the cellular level

X-Ray Microscopy Conference 2016

Schulz, G.^1 ; Götz, C.^1 ; Müller-Gerbl, M.^2 ; Zanette, I.^3 ; Zdora, M.-C.^3,4 ; Khimchenko, A.^1 ; Deyhle, H.^1 ; Thalmann, P.^1 ; Müller, B.^1

Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland^1

Musculoskeletal Research, Department of Biomedicine, University of Basel, Switzerland^2

Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, Didcot

OX11 0DE, United Kingdom^3

Department of Physics and Astronomy, University College London, London

WC1E 6BT, United Kingdom^4

关键词: Diamond light source;    Hierarchical imaging;    Highest resolutions;    Micrometer levels;    Multi-modal imaging;    Phase contrasts;    Spatial resolution;    Three dimensional visualization;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012026/pdf
DOI  :  10.1088/1742-6596/849/1/012026

来源: IOP

【 摘 要 】

Computed tomography reaches the best spatial resolution for the three-dimensional visualization of human tissues among the available nondestructive clinical imaging techniques. Nowadays, sub-millimeter voxel sizes are regularly obtained. Regarding investigations on true micrometer level, lab-based micro-CT (μCT) has become gold standard. The aim of the present study is firstly the hierarchical investigation of a human knee post mortem using hard X-ray μCT and secondly a multimodal imaging using absorption and phase contrast modes in order to investigate hard (bone) and soft (cartilage) tissues on the cellular level. After the visualization of the entire knee using a clinical CT, a hierarchical imaging study was performed using the lab-system nanotom® m. First, the entire knee was measured with a pixel length of 65 μm. The highest resolution with a pixel length of 3 μm could be achieved after extracting cylindrically shaped plugs from the femoral bones. For the visualization of the cartilage, grating-based phase contrast μCT (I13-2, Diamond Light Source) was performed. With an effective voxel size of 2.3 μm it was possible to visualize individual chondrocytes within the cartilage.