Determination of carbon-to-nitrogen ratio in the filamentous and heterocystous cyanobacterium Anabaena sp. PCC 7120 with single-cell soft X-ray imaging

X-Ray Microscopy Conference 2016

Teramoto, T.^1 ; Yoshimura, M.^2 ; Azai, C.^3 ; Terauchi, K.^3 ; Ohta, T.^2

College of Science and Engineering, Ritsumeikan University, Shiga

525-8577, Japan^1

SR Center, Ritsumeikan University, Shiga

525-8577, Japan^2

College of Life Sciences, Ritsumeikan University, Shiga

525-8577, Japan^3

关键词: Carbon-to-nitrogen ratio;    Cyanobacterium anabaena;    Heterocystous cyanobacterium;    Heterocysts;    Nitrogen K-edge;    Single cells;    Soft x-ray microscopy;    Vegetative cells;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012005/pdf
DOI  :  10.1088/1742-6596/849/1/012005

来源: IOP

【 摘 要 】

Vegetative cells and heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120 were observed by soft X-ray microscopy. Carbon-to-nitrogen (C/N) ratio of each cell was estimated by the difference of the absorbance of the images below and above the nitrogen K-edge absorption. It was revealed that the C/N ratios in vegetative cells and heterocysts are 4.54 and 2.46, respectively.