Two-photon luminescence and stimulated emission depletion with gold nanorods by a single wavelength

6th conference on Advances in Optoelectronics and Micro/nano-optics

Chen, Jianling^1 ; You, Minghai^1 ; Peng, Yiru^2 ; Yang, Hongqin^1 ; Xie, Shusen^1

Key Laboratory of Optoelectronic Science and Technology, Medicine of Ministry of Education, Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou

350007, China^1

College of Chemistry and Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou

350007, China^2

关键词: Fluorescent dyes;    Optimal wavelength;    Single wavelength;    Single wavelength lasers;    STED microscopies;    Stimulated emission depletion;    Two-photon autofluorescence;    Two-photon luminescence;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/844/1/012064/pdf
DOI  :  10.1088/1742-6596/844/1/012064

来源: IOP

【 摘 要 】

Single-wavelength two photon STED technique simplifies common STED setup, and decreases the cost of the system. However, this technique limits the fluorescent dye to a few kinds. Because the dye should be excited and depleted by the same single wavelength laser. And the gold nanorods can be excited by near-infrared pulsed laser and produces two photon luminescence, which is brighter than general two-photon autofluorescence by three orders. We found that the gold nanorods can also be depleted by near-infrared pulsed laser. We studied the stimulated emission depletion of gold nanorods with a single wavelength laser in the near infrared. Two photon luminescence was excited with a femtosecond pulse, then depleted by stimulated emission with a stretched pulse. The depletion efficiency with different wavelength was measured to pick out the optimal wavelength. We showed that this method can be applied to super-resolved STED microscopy, which combines the high brightness of the nanorods and the simplicity of the single-wavelength STED system.