Dual-color super-resolution imaging by fluorescence emission depletion (STED) microscopy

6th conference on Advances in Optoelectronics and Micro/nano-optics

Wang, Wen-Sheng^1 ; Kuang, Cui-Fang^1 ; Liu, Shao-Cong^1 ; Sun, Shi-Yi^1 ; Liu, Xu^1

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou

310027, China^1

关键词: Biological samples;    Design and build;    Dual color;    Fluorescence emission;    Lateral resolution;    Stimulated emission depletion microscopy;    Super resolution imaging;    Two-color;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/844/1/012033/pdf
DOI  :  10.1088/1742-6596/844/1/012033

来源: IOP

【 摘 要 】

We design and build a stimulated emission depletion (STED) microscopy which is capable of super-resolution imaging in two colors. The enhancement in the lateral resolution is demonstrated experimentally through fluorescent beads and biological samples. The dual-color modality enables the system to be suitable for further biological investigations.