Additive interaction of carbon dots extracted from soluble coffee and biogenic silver nanoparticles against bacteria

5th Nanosafe International Conference on Health and Safety issues related to nanomaterials for a socially responsible approach

物理学;医药卫生;材料科学

Andrade, Patricia F.^1 ; Nakazato, Gerson^2 ; Durán, Nelson^1,3,4

Institute of Chemistry, University of Campinas (UNICAMP), Campinas

SP, Brazil^1

Biology Sciences Center, Londrina State University (UEL), Londrina, Brazil^2

NanoBioss, Institute of Chemistry, UNICAMP, Campinas

SP, Brazil^3

Brazil. Nat. Nanotechnol. Lab. (LNNano-CNPEM), Campinas

SP, Brazil^4

关键词: Antibacterial compounds;    Average size;    Coffee grounds;    Coffee powders;    FT-IR spectrum;    Inhibitory concentration;    Soluble coffee;    Well-dispersed;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012028/pdf
DOI  :  10.1088/1742-6596/838/1/012028

来源: IOP

【 摘 要 】

It is known the presence of carbon dots (CDs) in carbohydrate based foods. CDs extracted from coffee grounds and instant coffee was also published. CDs from soluble coffee revealed an average size of 4.4 nm. CDs were well-dispersed in water, fluorescent and we have characterized by XPS, XRD analysis, fluorescence and by FTIR spectra. The MIC value by serial micro-dilution assays for CDs on S. aureus ATCC 25923 was 250 μg/mL and E. coli ATCC 25922 >1000 ug/mL. For silver nanoparticles biogenically synthesized was 6.7 μg/mL. Following the checkerboard assay with combining 1/2 MIC values of the MICs of 125 μg/mL of carbon dots and 3.4 μg/mL of silver nanoparticles, following the fractionated inhibitory concentration (FIC) index methodology, on S. aureus gave a fractionated inhibitory concentration (FIC) value of 1.0, meaning additive interaction. In general, the unfunctionalized CDs showed to be inefficient as antibacterial compounds, however the CDs extracted from Coffee powder and together silver nanoparticles appeared interesting as antibacterial association.