In vitro human digestion test to monitor the dissolution of silver nanoparticles

5th Nanosafe International Conference on Health and Safety issues related to nanomaterials for a socially responsible approach

物理学;医药卫生;材料科学

Bove, P.^1 ; Malvindi, M.A.^2 ; Sabella, S.^1

Istituto Italiano di Tecnologia, Drug Discovery and Development Department, Via Morego, 30, Genova

16136, Italy^1

Istituto Italiano di Tecnologia, Center for Bio-Molecular Nanotechnologies at UniLe, Via Barsanti, Arnesano (Lecce)

73010, Italy^2

关键词: Biological fluids;    Dissolution behaviour;    Food contact material;    Food industries;    Gastrointestinal tract;    Human digestion;    Nanoparticle suspension;    Scientific revolutions;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012003/pdf
DOI  :  10.1088/1742-6596/838/1/012003

来源: IOP

【 摘 要 】

Nanotechnology is a scientific revolution that the food industry has experienced over the last years. Widely employed as food additives and/or food contact materials in consumer products, silver nanoparticles are an example of this innovation. However, their increasing use makes also likely the human ingestion, thus requiring a proper risk analysis. In this framework, a comprehensive characterization of biotransformation of silver nanoparticles in biological fluids is fundamental for the regulatory needs. Herein, we aimed at studying the dissolution behaviour of silver nanoparticles using an in vitro test, which simulates the human oral ingestion of NPs during their passage through the gastrointestinal tract. The nanoparticle suspensions were characterized in the different digestion phases using several techniques to follow the changes of key physical properties (e.g., size, surface charge and plasmon peak) and to quantify the biotransformed products arisen by the process, as for example free silver ions.