Optimisation of Deacetylation Process for Chitosan Production from Red Snapper (Lutjanus sp.) Scale Wastes

International Seminar on Mathematics, Science, and Computer Science Education 2016

数学;自然科学;计算机科学

Takarina, N.D.^1 ; Indah, A.B.^1 ; Nasrul, A.A.^1 ; Nurmarina, A.^1 ; Saefumillah, A.^2 ; Fanani, A.A.^1 ; Loka, K.D.P.^1

Marine Science Postgraduate Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, West Java

16424, Indonesia^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, West Java

16424, Indonesia^2

关键词: Deacetylation;    Degree of deacetylation;    Deproteination;    Effective concentration;    Heating treatments;    Marine products;    Nitrogen content;    Optimisations;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/812/1/012110/pdf
DOI  :  10.1088/1742-6596/812/1/012110

来源: IOP

【 摘 要 】

Red snapper (Lutjanus sp.) is common tropical fish that known as important source of marine product in particular Indonesia. This research aimed to optimise the chitosan synthesis from the red snapper scale waste through deacetylation process. Method in this research was divided into three stages which were deproteination, demineralization, and deacetylation. Deproteination stage was done with solution containing 4.2% w/v NaOH and heated at 60° C for 5 hours and followed by the demineralization stage with solution containing 52% v/v 2 N HCl at room temperature for 6 hours. The comparison between fish scales and solutions was 1: 6. After that, process continued with the deacetylation. Several treatment during the deacetylation process were taken into consideration to determine the effective concentration for yielding optimum chitosan output. Chitosan produced were having moisture content of 2.88%, ash content of 1.10%, and nitrogen content of 0.0136%. Optimal Degree of Deacetylation (DDA) was up to 90.83% that obtained by heating treatment at a temperature of 110° C with solution containing 80% NaOH for 4 hours, and comparison between chitin : solution was 1 : 3. This result indicated that chitosan extracted from red snapper scale is very potential and can be applied to industry.