The Bacterial Endospore Stain on Schaeffer Fulton using Variation of Methylene Blue Solution

International Seminar on Mathematics, Science, and Computer Science Education 2016

数学;自然科学;计算机科学

Oktari, A.^1 ; Supriatin, Y.^1 ; Kamal, M.^1 ; Syafrullah, H.^2

Department of Medical Analyst, School of Analyst Bakti Asih, Padasuka Street No. 233, Pasirlayung, Bandung

40192, Indonesia^1

Medical School of Dharma Husada, Jakarta Street No. 75, Antapani, Bandung

40282, Indonesia^2

关键词: Bacterial endospores;    Bacterial spore;    Malachite green;    Methylene blue solution;    pH variation;    Vegetative cells;    Wall thickness;    Warm-up time;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/812/1/012066/pdf
DOI  :  10.1088/1742-6596/812/1/012066

来源: IOP

【 摘 要 】

Endospores staining is the type of staining to recognize the presence spore in bacterial vegetative cells. The bacterial endospores need a staining which can penetrate wall thickness of spore bacteria. A method of endospores staining is Schaeffer Fulton method that used Malachite Green. It is an alkaline substance staining that can staining the spore bacteria. In this research, it have found the alternative staining that can replace Malachite Green solution in spore bacterial stain. The alternative staining used is Methylene Blue solution (0,5 %, 0,7%, and 1% concentration) with pH variation (10, 11, and 12), and varyous heating time (3, 4, and 5 minutes). The all treatments staining have been effect on bacterial spores staining results. The warming time greatly affect the dye to penetrate the walls of bacterial spores, this can be seen in the results with various concentration at pH 10, indicates that the not long warm-up time 3 and 4 minutes, bacterial spores are not stained, while in the longer heating time is 5 minutes bacterial spores stained. This is caused because the longer heating time can make the pores of spore wall is open so that can facilitate the dye to get into the bacterial spores.