Computer processing of large datasets in the diagnosis of cancer micrometastases in the bone marrow

International Conference on Particle Physics and Astrophysics

物理学;天文学

Burov, D.A.^1 ; Selchuk, V.Y.^1,2 ; Beznos, O.A.^1 ; Obarevich, E.S.^1 ; Tupitsyn, N.N.^1

N.N. Blokhin Russian Cancer Research Center, Kashirskoe highway 23, Moscow

115478, Russia^1

National Research Nuclear University MEPhI, Moscow Engineering Physics Institute, Kashirskoe highway 31, Moscow

115409, Russia^2

关键词: Acoustic focusing;    Computer processing;    Emission spectrums;    Flow cytometers;    Fluorochromes;    Large datasets;    Sequential logic;    Solid tumors;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/798/1/012142/pdf
DOI  :  10.1088/1742-6596/798/1/012142

学科分类：天文学(综合)

来源: IOP

【 摘 要 】

To detect disseminated tumor cells (DTC) in the bone marrow in patients with solid tumors by flow cytometry it is necessary to analyze a very large number of cells (up to 100 million myelokaryocytes). We have proposed the use of a new generation flow cytometers with acoustic focusing, making it possible to analyze up to 20 mln events in one file. To improve the accuracy of our analysis we used fluorochromes with non-overlapping emission spectra, which makes it possible to minimize the need of setting compensation of cytometer. Identification of the target cell population detected by sequential logic gating.