Spectrophotometric method for quantitative measuring essential oil in aromatic water and distillate with rose smell

1st International Symposium 'Physics, Engineering and Technologies for Bio-Medicine'

医药卫生;物理学;工业技术

Semenova, E.^1 ; Presnyakova, V.^2 ; Goncharov, D.^3 ; Goncharov, M.^3 ; Presnyakova, E.^4 ; Presnyakov, S.^5 ; Moiseeva, I.^1 ; Kolesnikova, S.^6

Penza State University, Ministry of Education and Science of the Russian Federation, Penza

440026, Russia^1

Academic Gymnasium 1534, Moscow

117036, Russia^2

Province Boarding School for Gifted Children, Penza

440046, Russia^3

State Commission of Russian Federation for Selection Achievements Test and Protection, Moscow

107139, Russia^4

Bauman Moscow State Technical University, Moscow

105005, Russia^5

National Research Nuclear University, MEPhI (Moscow Engineering Physics Institute), Moscow

115409, Russia^6

关键词: Aromatic substances;    Different origins;    Hydrodistillations;    Monoterpene alcohols;    Oil concentration;    Spectro-photometric method;    Standard solutions;    Water and steams;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/784/1/012053/pdf
DOI  :  10.1088/1742-6596/784/1/012053

来源: IOP

【 摘 要 】

In this connection, we improved the express methods of determining the mixture of volatile aromatic substances by the spectrophotometry of aromatic water and steam distillate of essential oil raw materials (traditional or biotechnological with rose smell). Direct spectrophotometry of distillation water is impossible because it is a colloid of liquid oil and law is not observed. Therefore, it is necessary to dissolve 1 ml of distillate in ethanol in the ratio 1:4, in this case we take real solution with no lipophilic fall-out on the walls of cuvette, also the light absorption law is observed. There are stable maximums in spectrums of studied oils. Optical density of these maximums is a result of summary absorption of terpenoid components (aromatic and monoterpene alcohols, its ethers). Optical density of tested and standard solutions is measured in appropriate wavelengths. Spectrophotometric method of determination of essential oil quantity in aromatic water with rose smell differs with high sensitivity (10-5-10-6gmol/l) and allows to determine oil concentration from 0,900 to 0,008 mg with an error less than 1%. At that, 1 ml is enough for analysis. It's expedient to apply this method while operating with small quantity of water distillate in biochemical and biotechnological researches and also as express control for extraction and hydrodistillation of essential oil raw material (rose petals and flowers from different origin, eremothecium cultural liquid etc.).