Improvement of antigen detection efficiency with the use of two-dimensional photonic crystal as a substrate

1st International Symposium 'Physics, Engineering and Technologies for Bio-Medicine'

医药卫生;物理学;工业技术

Dovzhenko, Dmitriy^1,2 ; Terekhin, Vladimir^1 ; Vokhmincev, Kirill^1 ; Sukhanova, Alyona^1,3 ; Nabiev, Igor^1,3

Laboratory of Nano-Bioengineering, Russia^1

Department of the Physics of Micro- and Nanosystems, National Research Nuclear University, MEPhI (Moscow Engineering Physics Institute), Moscow, Russia^2

Laboratoire de Recherche en Nanosciences, LRN-EA4682, Université de Reims Champagne-Ardenne, Reims, France^3

关键词: Antigen detections;    Enzyme linked immunosorbent assay;    Fluorescent labels;    Fluorescent signals;    Multiplex detections;    Specific detection;    Two dimensional (2D) photonic crystals;    Two-dimensional photonic crystals;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/784/1/012018/pdf
DOI  :  10.1088/1742-6596/784/1/012018

来源: IOP

【 摘 要 】

Multiplex detection of different antigens in human serum in order to reveal diseases at the early stage is of interest nowadays. There are a lot of biosensors, which use the fluorescent labels for specific detection of analytes. For instance, common method for detection of antigens in human serum samples is enzyme-linked immunosorbent assay (ELISA). One of the most effective ways to improve the sensitivity of this detection method is the use of a substrate that could enhance the fluorescent signal and make it easier to collect. Two-dimensional (2D) photonic crystals are very suitable structures for these purposes because of the ability to enhance the luminescent signal, control the light propagation and perform the analysis directly on its surface. In our study we have calculated optimal parameters for 2D-dimensional photonic crystal consisting of the array of silicon nano-rods, fabricated such photonic crystal on a silicon substrate using reactive ion etching and showed the possibility of its efficient application as a substrate for ELISA detection of human cancer antigens.