Use of semiconductor nanocrystals to encode microbeads for multiplexed analysis of biological samples

1st International Symposium 'Physics, Engineering and Technologies for Bio-Medicine'

医药卫生;物理学;工业技术

Berestovoy, Mikhail A.^1 ; Bilan, Regina S.^1 ; Krivenkov, Victor^1 ; Nabiev, Igor^1,2 ; Sukhanova, Alyona^1,2

Laboratory of Nano-Bioengineering, National Research Nuclear University, MEPhI (Moscow Engineering Physics Institute), Moscow, Russia^1

Laboratoire de Recherche en Nanosciences, LRN - EA4682, Université de Reims Champagne-Ardenne, Reims, France^2

关键词: Biological markers;    Biological samples;    Charged polyelectrolytes;    Layer by layer deposition;    Luminescence intensity;    Multiplexed analysis;    Negatively charged;    Semiconductor nanocrystals;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/784/1/012012/pdf
DOI  :  10.1088/1742-6596/784/1/012012

来源: IOP

【 摘 要 】

Microbeads encoded with semiconductor quantum dots (QDs) are suitable tools for multiplexed analyses of various biological markers using flow cytometry. We have prepared a panel of microbeads encoded with QDs of different colors emitting with different luminescence intensities using the layer-by-layer deposition technique, which consists in layering of alternately charged polyelectrolytes and negatively charged QDs onto the surface of microbeads. This method allows QDs to be separated with one or several polymer layers in order to prevent Förster resonance energy transfer (FRET) and the resultant quenching of QD fluorescence in multicolor microbeads.