Single cell array impedance analysis in a microfluidic device

27th Micromechanics and Microsystems Europe Workshop

物理学;力学

Altinagac, Emre^1 ; Taskin, Selen^2 ; Kizil, Huseyin^2

Department of Nanoscience and Nanotechnology, Istanbul Technical University, Istanbul, Turkey^1

Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Istanbul, Turkey^2

关键词: Glass substrates;    Human embryonic kidneys;    Impedance analysis;    Inter-digitated electrodes;    Lab-on-a-chip devices;    Micro-fluidic devices;    PDMS microchannels;    Single cells;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/757/1/012010/pdf
DOI  :  10.1088/1742-6596/757/1/012010

学科分类：力学，机械学

来源: IOP

【 摘 要 】

Impedance analysis of single cells is presented in this paper. Following the separation of a target cell type by dielectrophoresis in our previous work, this paper focuses on capturing the cells as a single array and performing impedance analysis to point out the signature difference between each cell type. Lab-on-a-chip devices having a titanium interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to capture each cell in a single form and perform impedance analysis. HCT116 (homosapiens colon colorectal carcin) and HEK293 (human embryonic kidney) cells are used in our experiments.