3D super-resolution microscopy of bacterial division machinery

3rd International School and Conference on Optoelectronics, Photonics, Engineering and Nanostructures (Saint Petersburg OPEN 2016)

Vedyaykin, A.D.^1 ; Sabantsev, A.V.^1 ; Vishnyakov, I.E.^2 ; Morozova, N.E.^1 ; Polinovskaya, V.S.^1 ; Khodorkovskii, M.A.^1

Research Institute of Nanobiotechnologies, Peter the Great St.Petersburg Polytechnic University, Saint-Petersburg

195251, Russia^1

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av. 4, Saint Petersburg

194064, Russia^2

关键词: Complex structure;    Resolution limits;    Single-molecule localizations;    Super resolution;    Super-resolution microscopy;    Two Dimensional (2 D);   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/741/1/012066/pdf
DOI  :  10.1088/1742-6596/741/1/012066

来源: IOP

【 摘 要 】

Super-resolution microscopy is a promising tool for the field of microbiology, as bacteria sizes are comparable to the resolution limit of light microscopy. Bacterial division machinery and FtsZ protein in particular attract much attention of scientists who use different super-resolution microscopy techniques, but most of the available data on FtsZ structures was obtained using two-dimensional (2D) super-resolution microscopy. Using 3D single-molecule localization microscopy (SMLM, namely dSTORM) to visualize FtsZ, we demonstrate that this approach allows more accurate interpretation of super-resolution images and provides new opportunities for the study of complex structures like bacterial divisome.