Dissolution and mixing of flavin mononucleotide in microfluidic chips for bioassay

3rd International School and Conference on Optoelectronics, Photonics, Engineering and Nanostructures (Saint Petersburg OPEN 2016)

Belousov, K.I.^1,2 ; Denisov, I.A.^2 ; Lukyanenko, K.A.^2 ; Yakimov, A.S.^2 ; Bukatin, A.S.^2,3 ; Kukhtevich, I.V.^2 ; Sorokin, V.V.^2 ; Esimbekova, E.N.^2,4 ; Belobrov, P.I.^2,4 ; Evstrapov, A.A.^1,3,5

Department of Material Science and Nanotechnology, ITMO University, St. Petersburg

197101, Russia^1

Department of Biophysics, Siberian Federal University, Krasnoyarsk

660041, Russia^2

Nanobiotech Lab, St. Petersburg Academic University, St. Petersburg

194021, Russia^3

Laboratory of Photobiology, Institute of Biophysics SB RAS, Krasnoyarsk

660041, Russia^4

Laboratory of Information and Measurement Biosensor and Chemosensor Microsystems, Institute for Analytical Instrumentation RAS, St. Petersburg

198095, Russia^5

关键词: Analysis of liquids;    Concentration distributions;    Constant flow rates;    Flavin mono nucleotides (FMN);    Flavin mononucleotides;    Frequency of oscillation;    Uniform distribution;    Variable flow rate;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/741/1/012058/pdf
DOI  :  10.1088/1742-6596/741/1/012058

来源: IOP

【 摘 要 】

Dissolution and mixing of flavin mononucleotide (FMN), which activates a luminescent reaction, were considered in various designs of microfluidic chip for pollution analysis of liquid samples. The aim was to determine the velocity mode of fluid flow ensured the uniform distribution of the FMN in the reaction chamber. Simulation of concentration distribution of FMN in various designs of microfluidic chips was conducted. It was shown that the passive mixing techniques based on the constant flow rate didn't provide mixing of FMN in acceptable time (3 seconds). The most efficient mixing was achieved using variable flow rate with a gradually increasing frequency of oscillation.