Photophysical investigations of squaraine and cyanine dyes and their interaction with bovine serum albumin

India-Japan Expert Group Meeting on Biomolecular Electronics & Organic Nanotechnology for Environment Preservation

物理学;生物科学;生态环境科学

Saikiran, M.^1 ; Sato, D.^1 ; Pandey, S.S.^1 ; Kato, T.^1

Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Kitakyushu, Wakamatsu

808-0196, Japan^1

关键词: Associative interactions;    Binding constant;    Bovine serum albumins;    Phosphate buffer solutions;    Phosphate buffers;    Photophysical investigation;    Reactive functionality;    Strong interaction;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/704/1/012012/pdf
DOI  :  10.1088/1742-6596/704/1/012012

来源: IOP

【 摘 要 】

A model far-red sensitive symmetrical squaraine dye (SQ-3) and unsymmetrical near infra-red sensitive cyanine dye (UCD-1) bearing direct-COOH functionalized indole ring were synthesized, characterized and subjected to photophysical investigations including their interaction with bovine serum albumin (BSA) as a model protein in phosphate buffer solution (PBS). Both of the dyes exhibit strong interaction with BSA in phosphate buffer with high apparent binding constant. A judicious tuning of hydrophobic main backbone with reactive functionality for associative interaction with active site of BSA has been found to be necessary for BSA detection in PBS.