Morphological study of lipid vesicles in presence of amphotericin B via modification of the microfluidic CellASIC platform and LED illumination microscopy

INERA Conference 2015: Light in Nanoscience and Nanotechnology

Genova, J.^1 ; Decheva-Zarkova, M.^1 ; Pavli, J.I.^1

Institute of Solid State Physics, BAS, 72 Tzarigradsko Chaussee, Sofia

1784, Bulgaria^1

关键词: Experimental realizations;    Giant lipid vesicles;    Illumination sources;    Inverted phase contrast microscopies;    Irreversible effects;    Microfluidic platforms;    Morphological behavior;    Scientific investigation;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/682/1/012029/pdf
DOI  :  10.1088/1742-6596/682/1/012029

来源: IOP

【 摘 要 】

Giant lipid vesicles (liposomes) are the simplest model of the biological cell and can be easily formed from natural or synthetic lipid species with controlled composition and properties. This is the reason why they are the preferred objects for various scientific investigations. Amphotericin B (AmB) is a membrane active drug, used for treatment of systemic fungal infections. In this work we studied the morphological behavior of giant SOPC vesicles in asymmetrical presence of amphotericin B antibiotic in the vicinity of the lipid membrane. The visualization of the vesicles was carried out via inverted phase contrast microscopy. The illumination source was modified in a way that tungsten light bulb was replaced by 10 W white LED chip. All the experiments were performed using CellASIC ONIX Microfluidic Platform. The setup has been modified thus opening new opportunities for a variety of experimental realizations. The performed morphological studies showed strong and irreversible effect on the vesicle shape at the presence of amphotericin B in concentration 10-5g/l in the outer for the liposome's membrane solution. At concentration 10-3g/l AmB the effect was less visible and in 15-20 minutes the vesicles regained its initial spherical shape.