STEM electron tomography in the Scanning Electron Microscope

Electron Microscopy and Analysis Group Conference 2015

Ferroni, M.^1,3 ; Signoroni, A.^1 ; Sanzogni, A.^1 ; Sberveglieri, G.^1,3 ; Migliori, A.^2 ; Ortolani, L.^2 ; Christian, M.^2,4 ; Masini, L.^2 ; Morandi, V.^2

Department of Information Engineering, Brescia University, Via Valotti 9, Brescia

25123, Italy^1

CNR-IMM Section of Bologna, via Gobetti 101, Bologna

40129, Italy^2

CNR-INO, Via Valotti 9, Brescia

25123, Italy^3

CNR-ISOF, via Gobetti 101, Bologna

40129, Italy^4

关键词: Biological science;    Cobalt particles;    Collagen fibrils;    Electron tomography;    Projection image;    Structure reconstruction;    Tomographic reconstruction;    Transmission imaging;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/644/1/012012/pdf
DOI  :  10.1088/1742-6596/644/1/012012

来源: IOP

【 摘 要 】

The scanning-transmission imaging mode in the SEM allows for the threedimensional tomographic reconstruction of a specimen, starting from a set of projection images. Compressed sensing was used to solve the undetermined problem of structure reconstruction and was proven capable of overcoming the limitations arising from the sampling scheme. Reconstructions of cobalt particles within a carbon nanotube and collagen fibrils in a dermal tissue are presented, demonstrating the potential of this technique in the set of 3-D electron microscopy methods for both physical and biological science.