Molecular Beacon CNT-based Detection of SNPs

2nd International School and Conference Saint-Petersburg OPEN on Optoelectronics, Photonics, Engineering and Nanostructures

Egorova, V.P.^1 ; Krylova, H.V.^2 ; Lipnevich, I.V.^2 ; Veligura, A.A.^1 ; Shulitsky, B.G.^3 ; Fedotenkova, L.Y.^1

Belarusian State Pedagogical University, 18 Sovetskaya Str, Minsk

200005, Belarus^1

Physics Department, Belarusan State University, 4 Nezavisimosti Ave., Minsk

200030, Belarus^2

Belarusian State University of Informatics and Radioelectronics, 6 P. Brovki Str, Minsk

200013, Belarus^3

关键词: Carboxyl groups;    Chemically modified;    Few-walled carbon nanotubes;    Fluorescence quenching effect;    Molecular beacon;    Nucleotide basis;    Single-nucleotide polymorphisms;    Stacking interaction;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/643/1/012023/pdf
DOI  :  10.1088/1742-6596/643/1/012023

来源: IOP

【 摘 要 】

An fluorescence quenching effect due to few-walled carbon nanotubes chemically modified by carboxyl groups has been utilized to discriminate Single Nucleotide Polymorphism (SNP). It was shown that the complex obtained from these nanotube and singlestranded primer DNA is formed due to stacking interactions between the hexagons of the nanotubes and aromatic rings of nucleotide bases as well as due to establishing of hydrogen bonds between acceptor amine groups of nucleotide bases and donor carboxyl groups of the nanotubes. It has been demonstrated that these complexes may be used to make highly effective DNA biosensors detecting SNPs which operate as molecular beacons.