Multispectral digital lensless holographic microscopy: from femtosecond laser to white light LED

23rd Congress of the International Commission for Optics

Garcia-Sucerquia, J.^1

Universidad Nacional de Colombia, Sede Medellin, School of Physics, A.A. 3840, Medellin

050034, Colombia^1

关键词: Biological samples;    Biological specimens;    Broadband emission;    Broadband Light Sources;    Competitive performance;    Drosophila melanogaster;    Lensless holographic microscopies;    Multispectral imaging;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/605/1/012011/pdf
DOI  :  10.1088/1742-6596/605/1/012011

来源: IOP

【 摘 要 】

The use of femtosecond laser radiation and super bright white LED in digital lensless holographic microscopy is presented. For the ultrafast laser radiation two different configurations of operation of the microscope are presented and the dissimilar performance of each one analyzed. The microscope operating with a super bright white light LED in combination with optical filters shows very competitive performance as it is compared with more expensive optical sources. The broadband emission of both radiation sources allows the multispectral imaging of biological samples to obtain spectral responses and/or full color images of the microscopic specimens; sections of the head of a Drosophila melanogaster fly are imaged in this contribution. The simple, solid, compact, lightweight, and reliable architecture of digital lensless holographic microscopy operating with broadband light sources to image biological specimens exhibiting micrometer-sized details is evaluated in the present contribution.