Matrix effect on the analysis of amphenicols in fish by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

7th Brazilian Congress on Metrology

Guidi, L.R.^1 ; Tette, P.A.S.^1 ; Evangelista, W.P.^1 ; Fernandes, C.^2 ; Glória, M.B.A.^1

LBqA-Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte MG

31270-901, Brazil^1

Laboratório de Controle de Qualidade de Medicamentos, Faculdade de Farmácia, UFMG, Av. Antônio Carlos, Belo Horizonte, MG

31270-901, Brazil^2

关键词: Analytes;    Calibration curves;    Fish samples;    Florfenicol;    Liquid chromatography tandem mass spectrometry (LC MS/MS);    Matrix effects;    Quantitative determinations;    Thiamphenicol;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/575/1/012036/pdf
DOI  :  10.1088/1742-6596/575/1/012036

来源: IOP

【 摘 要 】

Matrix effect is an important parameter to be investigated during the development and validation of a method for the quantitative determination of contaminants in food. The objective of this study was to evaluate the matrix effect, through statistical tests, in the quantification of amphenicols in fish by HPLC-MS/MS. The study was performed by comparing the standard curves prepared in solvent solutions and in a fish sample previously known to be free of amphenicols. Since matrix effect was observed for the three analytes, calibration curves for quantification of chloramphenicol, thiamphenicol and florfenicol should be constructed using the matrix.