Uncertainty assessment in measurement of myotube thickness in cells culture treated with and without therapeutic ultrasound

7th Brazilian Congress on Metrology

Abrunhosa, V.M.^1,2 ; Costa, M.L.^2 ; Mermelstein, C.^2 ; Alvarenga, A.V.^1 ; Costa-Félix, R.P.B.^1

Laboratory of Ultrasound, Direc. of Scientific and Industrial Metrology (DIMCI), National Institute of Metrology, Quality and Technology (Inmetro), Av. Nossa Sra das Graças, 50, Duque de Caxias

RJ, ZIP 25250-020, Brazil^1

Laboratory of Muscle Differentiation and Cytoskeleton, Biomedical Sciences Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro

ZIP 21949-590, Brazil^2

关键词: Control groups;    Myotubes;    Primary cell cultures;    Spatial differences;    Therapeutic ultrasound;    Ultrasound treatments;    Uncertainty assessment;    Uncertainty modeling;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/575/1/012002/pdf
DOI  :  10.1088/1742-6596/575/1/012002

来源: IOP

【 摘 要 】

Effectiveness of an ultrasound treatment shall be assessed by experiments. A reliable cell culture protocol is available and spatial discrepancies could arise. To assure if the spatial difference are relevant or not, and how they should be dealt with, an uncertainty model for the treatment result is a metrological reliable solution. The present work reports a metrological approach to assess myotube thickness and to validate a primary cell culture of muscle after a therapeutic ultrasound treatment, comparing it with a control group. The results reinforced the importance of such approach and show an efficacy of treatment on myotube differentiation.