Sample preparation and EFTEM of Meat Samples for Nanoparticle Analysis in Food

Electron Microscopy and Analysis Group Conference 2013

Lari, L.^1,2 ; Dudkiewicz, A.^3,4

Department of Physics, University of York, Heslington, YO10 5DD York, United Kingdom^1

York JEOL Nanocentre, York Science Park, Heslington, YO10 5BR York, United Kingdom^2

Food and Environment Research Agency, YO41 1LZ York, United Kingdom^3

Department of Environment, University of York, Heslington, YO10 5DD, United Kingdom^4

关键词: Analytical methodology;    Energy filtered TEM;    Food contact material;    Personal care products;    Reproducibilities;    Sample concentration;    Sample preparation;    TEM sample preparation;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/522/1/012057/pdf
DOI  :  10.1088/1742-6596/522/1/012057

来源: IOP

【 摘 要 】

Nanoparticles are used in industry for personal care products and the preparation of food. In the latter application, their functions include the prevention of microbes' growth, increase of the foods nutritional value and sensory quality. EU regulations require a risk assessment of the nanoparticles used in foods and food contact materials before the products can reach the market. However, availability of validated analytical methodologies for detection and characterisation of the nanoparticles in food hampers appropriate risk assessment. As part of a research on the evaluation of the methods for screening and quantification of Ag nanoparticles in meat we have tested a new TEM sample preparation alternative to resin embedding and cryo-sectioning. Energy filtered TEM analysis was applied to evaluate thickness and the uniformity of thin meat layers acquired at increasing input of the sample demonstrating that the protocols used ensured good stability under the electron beam, reliable sample concentration and reproducibility.