Synchrotron radiation X-ray microtomography and histomorphometry for evaluation of chemotherapy effects in trabecular bone structure

22nd International Congress on X-Ray Optics and Microanalysis

Alessio, R.^1 ; Nogueira, L.P.^2 ; Almeida, A.P.^1 ; Colaço, M.V.^2 ; Braz, D.^1 ; Andrade, C.B.V.^3 ; Salata, C.^3 ; Ferreira-Machado, S.C.^4 ; De Almeida, C.E.^3 ; Tromba, G.^5 ; Barroso, R.C.^2

COPPE-Universidade Federal Do Rio de Janeiro, PO Box 68509, 21945-970, Rio de Janeiro, Brazil^1

Laboratório de Física Médica, Universidade Do Estado Do Rio de Janeiro, Rua São Francisco Xavier, 524 PJLF, Rio de Janeiro, sl 3007F, Brazil^2

Laboratório de Ciências Radiológicas, Universidade Do Estado Do Rio de Janeiro, Rua São Francisco Xavier, 524 PHLC, Rio de Janeiro, sl 136, Brazil^3

Departamento de Biologia Geral, Universidade Federal Fluminense, Outeiro de São João Batista, s/n, Niterói, Brazil^4

Sincrotrone Trieste ScpA, Strada Statale S.S., 34012 Basovizza, Trieste, Italy^5

关键词: Laboratory animals;    Microcomputed tomography;    Morphometric parameters;    Premenopausal women;    Synchrotron radiation x-rays;    Tomographic images;    Trabecular bone structures;    Very high resolution;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/499/1/012019/pdf
DOI  :  10.1088/1742-6596/499/1/012019

来源: IOP

【 摘 要 】

Three-dimensional microtomography has the potential to examine complete bones of small laboratory animals with very high resolution in a non-invasive way. One of the side effects caused by some chemotherapy drugs is the induction of amenorrhea, temporary or not, in premenopausal women, with a consequent decrease in estrogen production, which can lead to bone changes. In the present work, the femur heads of rats treated with chemotherapy drugs were evaluated by 3D histomorphometry using synchrotron radiation microcomputed tomography. Control animals were also evaluated for comparison. The 3D tomographic images were obtained at the SYRMEP (SYnchrotron Radiation for MEdical Physics) beamline at the Elettra Synchrotron Laboratory in Trieste, Italy. Results showed significant differences in morphometric parameters measured from the 3D images of femur heads of rats in both analyzed groups.