X-ray fluorescence microscopy of light elements in cells: self-absorption correction by integration of compositional and morphological measurements

11th International Conference on X-ray Microscopy

Malucelli, E.^1 ; Iotti, S.^1,2 ; Fratini, M.^3,8 ; Marraccini, C.^9 ; Notargiacomo, A.^3 ; Gianoncelli, A.^4 ; Bukreeva, I.^3 ; Cedola, A.^3 ; Maier, J.^5 ; Farruggia, G.^1,2 ; Cappadone, C.^1 ; Merolle, L.^1 ; Wolf, F.^6 ; Trapani, V.^6 ; Lagomarsino, S.^7

Dipartimento di Farmacia e Biotecnologie, University di Bologna, Italy^1

Istituto Nazionale Biostrutture e Biosistemi, Rome, Italy^2

Institute of Photonics and Nanotechnology-CNR, Rome, Italy^3

Sincrotrone Trieste, Area Science Park, Basovizza Trieste, Italy^4

Dipartimento di Scienze Bionediche e Cliniche L. Sacco, University di Milano, Milan, Italy^5

Istituto Patologia Generale, University Cattolica Del Sacro Cuore, Rome, Italy^6

Institute Chemical and Physical Processess, C/o Phys. Dpt., University Sapienza, Rome, Italy^7

Fermi Center, Piazzale del Viminale 1, 00187 Rome, Italy^8

Dipartimento di Scienze della Vita, University di Modena e Reggio Emilia, Modena, Italy^9

关键词: Light elements;    Morphological information;    Morphological measurements;    Quantitative mapping;    Scanning transmission x ray microscopy;    Self absorption;    Structural information;    X-ray fluorescence microscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012022/pdf
DOI  :  10.1088/1742-6596/463/1/012022

来源: IOP

【 摘 要 】

We present here a new methodology for quantitative mapping of light elements in cells, based on combination of compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Microscopy and Scanning Transmission X-ray Microscopy (STXM). Since XRFM of light elements (carbon, nitrogen, oxygen, sodium and magnesium), are strongly influenced by self-absorption, we developed an algorithm to correct for this effect, using the morphological and structural information provided by AFM and STXM. Finally, the corrected distributions have been obtained, thus allowing quantitative mapping.