A compact Laboratory Transmission X-ray Microscope for the water window

11th International Conference on X-ray Microscopy

Legall, H.^1,2,3 ; Stiel, H.^3 ; Blobel, G.^3 ; Seim, C.^2 ; Baumann, J.^2 ; Yulin, S.^4 ; Esser, D.^5 ; Hoefer, M.^5 ; Wiesemann, U.^6 ; Wirtz, M.^6 ; Schneider, G.^7 ; Rehbein, S.^7 ; Hertz, H.M.^8

Berlin Laboratory for Innovative X-ray Technologies (BLiX), Germany^1

Technische Universität Berlin, Institut für Optik und Atomare Physik, Hardenbergstr. 36, D-10623 Berlin, Germany^2

Max-Born-Institut, Max-Born-Str. 2A, D-12489 Berlin, Germany^3

Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF, Beutenberg Campus, Albert-Einstein-Str. 7, D-07745 Jena, Germany^4

Fraunhofer-Institut für Lasertechnik ILT, Steinbachstr. 15, D-52074 Aachen, Germany^5

Bruker ASC GmbH, Waltherstrasse 49-51, D-51069 Köln, Germany^6

Helmholtz-Zentrum Berlin, Institute for Soft Matter and Functional Materials, Albert-Einstein-Str. 15, D-12489 Berlin, Germany^7

KTH (Royal Institute of Technology), Center Biomedical and X-ray Physics, Roslagstullsbacken 21, SE-106 91 Stockholm, Sweden^8

关键词: Biological specimens;    High-power laser systems;    Laboratory environment;    Natural environments;    Organic materials;    Plasma generation;    State of the art;    Transmission x-rays;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012013/pdf
DOI  :  10.1088/1742-6596/463/1/012013

来源: IOP

【 摘 要 】

In the water window (2.2-4.4 nm) the attenuation of radiation in water is significantly smaller than in organic material. Therefore, intact biological specimen (e.g. cells) can be investigated in their natural environment. In order to make this technique accessible to users in a laboratory environment a Full-Field Laboratory Transmission X-ray Microscope (L-TXM) has been developed. The L-TXM is operated with a nitrogen laser plasma source employing an InnoSlab high power laser system for plasma generation. For microscopy the Lyαemission of highly ionized nitrogen at 2.48 nm is used. A laser plasma brightness of 5 × 1011photons/(s × sr × μm2in line at 2.48 nm) at a laser power of 70 W is demonstrated. In combination with a state-of-the-art Cr/V multilayer condenser mirror the sample is illuminated with 106photons/(μm2× s). Using objective zone plates 35-40 nm lines can be resolved with exposure times