Direct mapping of 19F in 19FDG-6P in brain tissue at subcellular resolution using soft X-ray fluorescence

11th International Conference on X-ray Microscopy

Poitry-Yamate, C.^1,4 ; Gianoncelli, A.^2 ; Kourousias, G.^2 ; Kaulich, B.^3 ; Lepore, M.^1,4 ; Gruetter, R.^1,4,5 ; Kiskinova, M.^2

Biomedical Imaging Center, Ecole Polytechnique Fédérale de Lausanne, Switzerland^1

ELETTRA-Sincrotrone Trieste S.C.p.A., Trieste, Italy^2

Diamond Light Source Ltd, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom^3

Radiology Department, University of Lausanne, Switzerland^4

Radiology Department, University of Geneva, Switzerland^5

关键词: Cerebral glucose metabolisms;    Formalin fixation;    Hypothalamic neuron;    Intracellular domain;    Physiological mechanisms;    Spatial resolution;    Subcellular resolution;    Transmission x-rays;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012003/pdf
DOI  :  10.1088/1742-6596/463/1/012003

来源: IOP

【 摘 要 】

Low energy x-ray fluorescence (LEXRF) detection was optimized for imaging cerebral glucose metabolism by mapping the fluorine LEXRF signal of19F in19FDG, trapped as intracellular19F-deoxyglucose-6-phosphate (19FDG-6P) at 1μm spatial resolution from 3μm thick brain slices.19FDG metabolism was evaluated in brain structures closely resembling the general cerebral cytoarchitecture following formalin fixation of brain slices and their inclusion in an epon matrix. 2-dimensional distribution maps of19FDG-6P were placed in a cytoarchitectural and morphological context by simultaneous LEXRF mapping of N and O, and scanning transmission x-ray (STXM) imaging. A disproportionately high uptake and metabolism of glucose was found in neuropil relative to intracellular domains of the cell body of hypothalamic neurons, showing directly that neurons, like glial cells, also metabolize glucose. As19F-deoxyglucose-6P is structurally identical to18F- deoxyglucose-6P, LEXRF of subcellular19F provides a link to in vivo18FDG PET, forming a novel basis for understanding the physiological mechanisms underlying the18FDG PET image, and the contribution of neurons and glia to the PET signal.