Variations of Garlic Common Latent Virus and Shallot Latent Virus Concentration on Shallot and Garlic

2nd International Conference on Biosciences

Pauzi, Y.S.^1 ; Lestari, S.M.^1 ; Hidayat, S.H.^1

Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University Bogor Agricultural University, Kampus IPB Darmaga, Bogor

16680, Indonesia^1

关键词: Carlavirus;    Completely randomized designs;    Enzyme linked immunosorbent assay;    High incidence;    Indonesia;    Latent virus;    primary inoculum;    Primary sources;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/197/1/012045/pdf
DOI  :  10.1088/1755-1315/197/1/012045

来源: IOP

【 摘 要 】

Infection of Garlic common latent virus (GCLV) and Shallot latent virus (SLV) on shallot and garlic has been reported in Indonesia. Both viruses are known to infect the plants in the field in high incidence and can be transmitted through the bulbs. A research was conducted to determine the potency of infected bulbs as source of disease inoculum by measuring virus titer throughout bulb growing stadia. Two kinds of shallot's and garlic's bulbs was used, i.e. shallot seed bulb (cv. Bima curut), garlic seed bulb (cv. Sembalun), consumption shallot and garlic bulb (unknown cultivar). The experiment was conducted using completely randomized design. Ten samples with three repetition of each stadia, i.e. adventitious shoot, shoot, leaf on 7 days after planting (DAP), and 14 DAP were analyzed by enzyme-linked immunosorbent assay using specific antisera to GCLV and SLV. Infection of GCLV and SLV was found higher on garlic than on shallot. Both viruses were detected in high incidence from adventitious shoot and 14 DAP-foliar leaves. Virus titer is higher on consumption bulbs than those on seed bulbs for both shallot and garlic. This study indicated that infected bulbs is very potential as the primary source of disease in the field.