Phylogenetic Study of Genus Mangifera in Southern Sumatera Based on DNA Sequences of the Internal Transcribed Spacer (ITS) Region

2nd International Conference on Biosciences

Fitmawati^1 ; Ningrum, M.R.^1 ; Mahatma, R.^1 ; Suzanti, F.^2

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau Kampus Binawidya, Jl. HR. Soebrantas Km 12. 5, Pekanbaru, Riau, Indonesia^1

Department of Biology, Faculty of Education, Universitas Riau Kampus Binawidya, Jl. HR. Soebrantas Km 12. 5, Pekanbaru, Riau, Indonesia^2

关键词: Genetic distances;    Internal transcribed spacers;    Mangifera;    Maximum parsimony;    Molecular marker;    Phylogenetic analysis;    Phylogenetic studies;    Southern Sumatera;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/197/1/012013/pdf
DOI  :  10.1088/1755-1315/197/1/012013

来源: IOP

【 摘 要 】

The diversity of Mangifera in Southern Sumatra has not been thoroughly explored and studied. On the other hand, diversity of the existing Mangifera species is threatened with extinction due to the decline of their natural habitat in forest areas. This condition will automatically cause disappearance of Mangifera information, whereas the diversity has not been well identified and still being unclear upon its species stations. The purpose of this study was to reconstruct Mangifera relationship based on the sequences of nuclear ribosomal DNA (nrDNA) at the ITS region. DNA isolation of Mangifera leaf used CTAB method with slight modifications. DNA sequencing was conducted at First Base Laboratories, Malaysia. The reconstruction used PAUP program∗ Version 4.0b10 in both maximum parsimony (MP) and neighbor joining (NJ) methods. Cladogram by MP analysis formed a monophyletic clad with two main clads. The first clad consisted of M. foetida1, M. foetida2, M. foetida3, M. odorata, M. kemanga1, M. indica1, M. indica2, M. zeylanica and M. lalijiwa; and the second clad consisted of M. torquenda, M. quadrifides and M. kemanga2. Cladogram by NJ analysis strongly supported MP analysis and revealed that M.indica2 has the longest genetic distance compared to other species.