Construction of Surfactin--Polysorbate-80 reversed micelle systems and its application on extraction of cellulasee

2018 International Conference of Green Buildings and Environmental Management

土木建筑工程;生态环境科学

Peng, Xin^1 ; Tang, Yu^1 ; Xu, Peng-Fei^1 ; Du, Hao^1 ; Yuan, Lu^1 ; Meng, Yong^1

Natl. and Loc. U. Eng. Laboratory for New Petrochemical Materials and Fine Utilization of Resources, Key Laboratory of Resource Fine-Processing and Advanced Materials of Hunan Province, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, College of Chemistry and Chemical Engineering, Hunan Normal University Changsha, 410081, China^1

关键词: Backward extraction;    Forward-extraction;    Ionic surfactants;    ITS applications;    Micellar extraction;    Mixed reverse micelles;    Protein recovery;    Surfactant concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/186/2/012010/pdf
DOI  :  10.1088/1755-1315/186/2/012010

来源: IOP

【 摘 要 】

This work studied a novel method to construct a mixed reverse micelle system and its application in extracting and purifying enzymes. Mixed reversed micellar extraction formed by biosurfactant surfactin and ionic surfactant polysorbate-80 was applied for extracting cellulase. 40% surfactin and 60% polysorbate-80 were found to be the best system. The activity and protein recovery were obtained with the best conditions: in forward extraction, surfactant concentration was 3.0 mM; pH 7.0; temperature 40°C; KCl 0.05 M. In backward extraction, pH 7.5; temperature 40°C; KCl 0.1 M.