Modified Ganoderma selective medium to meet Indonesia's government regulation

INTERNATIONAL BIOTECHNOLOGY CONFERENCE ON ESTATE CROPS 2017

Amanda, Wiwit Iman^1 ; Prakoso, H.T.^2

Department of Agroecotechnology, Faculty of Agriculture, University of Brawijaya, Jalan Veteran 1, Malang

65145, Indonesia^1

Indonesian Research Institute for Biotechnology and Bioindustry, Jalan Taman Kencana No.1, Bogor 16128, Indonesia^2

关键词: Basal stems;    Ganoderma;    Government regulation;    Hazardous wastes;    Indonesia;    Malaysia;    Pentachloronitrobenzene (p CNB);    Selective medium;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/183/1/012020/pdf
DOI  :  10.1088/1755-1315/183/1/012020

来源: IOP

【 摘 要 】

Ganoderma boninense, the causal agent of basal stem rot in oil palm, has caused great losses on oil palm industry. Isolation method of the fungus from the field for further study is considered problematic due to high contaminations. To tackle this, Ganoderma selective medium (GSM) has been developed by scientists from Malaysia. The problem occurred when the media is going to be used in Indonesia due to the prohibition of one of its ingredients by Indonesia's government regulation No. 85/1995 on the management of hazardous wastes and toxic. This study aims to test whether the selective medium is still effective even without Pentachloronitrobenzene (PCNB) and Ridomil. The former is the prohibited ingredients and the latter is not well distributed in Indonesia thus considered as a rare item. The study showed that contamination can be kept to minimal and the distinguished feature of GSM, formation of a brown halo around the colony, was seen. However, the growth of Ganoderma's mycelium seems hampered. We hypothesize that besides being a fungicide, PCNB may play a role as a carbon source that supports growth for Ganoderma. The find for substitute ingredient of PCNB in GSM is on its way.