Microscopic callus selection of sengon tree (Falcataria moluccana) putative tolerant to Uromycladium falcatarium

INTERNATIONAL BIOTECHNOLOGY CONFERENCE ON ESTATE CROPS 2017

Putri, Asri Insiana^1

Centre for Forest Biotechnology and Tree Improvement, Jalan Palagan Tentara Pelajar KM 15, Purwobinangun, Pakem, Sleman Yogyakarta

55582, Indonesia^1

关键词: Biotic pathogens;    Cropping systems;    Good correlations;    Index values;    Living cell;    Selection in vitro;    Tolerance indices;    Viable cells;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/183/1/012006/pdf
DOI  :  10.1088/1755-1315/183/1/012006

来源: IOP

【 摘 要 】

The increase of the cultivation area of the sengon tree (Falcataria moluccana) through monoculture cropping system especially in Java has caused an increase in gall rust disease. Infestation of the disease may lead to 100% death of sengon seedlings. Uromycladium falcatarium has been identified as the fungus causing the gall rust epidemic. This research aimed to select calli putative tolerant to gall rust disease. Seeds of ten individual colonies of 3 clones of sengon trees from Purwobinangun district that have been attacked severely by gall rust were used as explant sources. Gall rust filtrate was used as bioagent selection in vitro and the viable calli were selected under a microscope. The callus viability was determined based on the average living cell percentage stained with fluorescence diacetic acids. These viable cells of callus were found to have a good correlation with the tolerance index value. The results showed that callus colony from clone 3 individual 4, clone 5 individual 5, and clone 6 individual 5 exhibited the highest tolerance index value. The cell with different index values to the biotic pathogen filtrate indicated the presence of an enzymatic defence process of the cells against gall rust attack.