Scaling up process for fish peptone production

2nd International Symposium on Green Technology for Value Chains 2017

工业技术;生态环境科学

Kosasih, W.^1 ; Ratnaningrum, D.^1 ; Endah, E.S.^1 ; Pudjiraharti, S.^1 ; Priatni, S.^1

Research Unit for Clean Technology, Indonesian Institute of Sciences (LIPI), Komplek LIPI Bandung, Jalan Sangkuriang, Gedung 50, Bandung

40135, Indonesia^1

关键词: Degree of hydrolysis;    Fish protein hydrolysate;    Microbial growth;    Nitrogen sources;    Outlet temperature;    Protease enzyme;    Protein hydrolysate;    Soluble proteins;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/160/1/012007/pdf
DOI  :  10.1088/1755-1315/160/1/012007

来源: IOP

【 摘 要 】

Fish peptone is one of protein hydrolysate products that can be used as nitrogen source for microbial growth. Peptone can be made from marine fish whereas protease enzyme was made from either papaya extract or commercial papain. Fish peptone is not only an eco-friendly approach but also can produce economic materials used in formulation of culture media. The objective of this research was to optimise the scaling up process of fish peptone production. An amount of 200 g of clean fish marine was blended with 1 L of water and added with 0.2 % of commercial papain at pH 8. Peptone production was carried out in a 1 L glass reactor with agitation (50, 100, 150, and 200 rpm) for 1-8 h. Fish slurry was then incubated at 50°C for 8 h. Fish protein hydrolysate was spray-dried with inlet and outlet temperature of 90°C and 80°C, respectively, at flow rate of 200 mL/h. Results revealed that the best condition for hydrolysis was shown by protein soluble content of 17.1 mg/L, α-amino content of 147 mM, and degree of hydrolysis of 8.3% which was achieved by agitation at 100 rpm for 8 h reaction. The yield of dried peptone product was 11.9 % (w/v). This peptone powder had higher soluble protein and α-amino content (442.3 mg/g, 124 mM) than the commercial peptone (258.5 mg/g, 65 mM). The microbial growth test of fish peptone products showed similar effectiveness compared with commercial bacterial peptone for the growth of Escherichia coli and Streptococcus aureus.